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Effect of DMSO on apoptosis and taxol biosynthesis in suspension cultured Taxus cuspidata cells

Ge Zhiqiang, Li Jingchuan, Yuan Yingjin, Hu Zongding

Department of Pharmaceutical Engineering,School of Chemical Engineering and Technology,Tianjin University.Tianjin 300072

Abstract

The results showed that 2% DMSO could decrease the cell viability significantly, suppress cell proliferation, and reduce the DNA content in the nucleus. Some of the cells treated with 2% DMSO showed typical apoptotic morphological changes under fluorescence microscope from the 8th to the 27th day of culture (Plate I-b,c,d). In the same time, the yield of taxol was enhanced evidently. These changes in the above description were not observed in the control group and the groups treated by DMSO of concentrations 0.5% and 1%. These findings show that DMSO at a certain concentration induces apoptosis and promotes taxol biosynthesis by Taxus cuspidata cells in suspension culture.

Key words: Taxes cuspidata cell;Dimethyl sulfoxide;Apoptosis;Cell viability;Taxol

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Citing This Article：

Ge Zhiqiang, Li Jingchuan, Yuan Yingjin, Hu Zongding. Effect of DMSO on apoptosis and taxol biosynthesis in suspension cultured Taxus cuspidata cells. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).