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Establishment of highly efficient regeneration system of maize

Wu Minsheng, Huang Jianqiu, Wei Zhiming

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The regeneration of maize immature embryo is related to the following factors: maize genetype, length of immature embryo, the time from budding to differentiation, the presence and concentration of 6-BA, IBA and Gelrite. Different maize varieties have different regeneration capacities, the best combination is that length of immature embryo is 1-2 mm, the time from buding to differentiation is 4-6 days, 6-BA is 0.5-0,6mg/L, IBA is 0.6-1.0mg/L for root growth. Gelrite could replace agar for maize rooting.

Key words: Maize;Immature embryo;Tissue culture;Plant regeneration

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Citing This Article：

Wu Minsheng, Huang Jianqiu, Wei Zhiming. Establishment of highly efficient regeneration system of maize. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).