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Sequence and function analysis of hupT gene in Rhodobacter sphaeroides 601

Xu Dongqing, Wu Yongqiang

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The hupT gene from hup cluster (cosmid I) in Rhodobacter sphaeroides was cloned and sequenced. It has been found to have a length of 1332 bp and to encode a 48.23 kD protein homologous to histidine kinase belonging to the superfamily of two component regulatory system. The HupT was overexpressed in E. coli and purified. The auto-phosphorylation of HupT showed that it was a histidine kinase. And the hupT gene could restore hydrogenase activity in R. capsulatus HupT mutant.

Key words: Rhodobacter sphaeroides;H_(2)-uptake hydrogenase;HupT gene;Histidine kinase

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Citing This Article：

Xu Dongqing, Wu Yongqiang. Sequence and function analysis of hupT gene in Rhodobacter sphaeroides 601. Acta Physiol Sin 2001; 53 (6): (in Chinese with English abstract).