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Isolation and cloning of cDNA related with the genes of Halonxylon ammodendron (Mey.) Bge. seedlings induced by osmotic stress by suppression subtractive hybridization

Guo Xinhong, Jiang Xiaocheng, Pan Xiaoling, Dai Yuchi, Jiang Weiming, Chen Liangbi

College of Life Science of Hunan Normal University.Changsha 410081,Hunan；China

Abstract

The results showed that the probes built from 3 candidate clones hybridized either only or more strongly with M total RNA, while the other 5 clones had no hybridization signals with H or M total RNA, which probably represented cDNA clones expressed by low-abundance transcripts in seedlings under osmotic stress. Sequences of the 3 positive cDNA clones were measured. By BLAST sequence similarity searching in Gene-Bank nucleotide sequence database, two cDNAs had the same sequence and shared a similarity of 98% to 100% with NaCl-specific cDNA other cDNA had some similarity with the cDNAs encoding senescence-associated genes from stored snow pea (Pisum sativum L. var. saccharatum) pods.

Key words: Suppression subtractive hybridization(SSH);H.ammodedron(Mey.) Bge;Seedlings;Osmotic stress;cDNA library

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Citing This Article：

Guo Xinhong, Jiang Xiaocheng, Pan Xiaoling, Dai Yuchi, Jiang Weiming, Chen Liangbi. Isolation and cloning of cDNA related with the genes of Halonxylon ammodendron (Mey.) Bge. seedlings induced by osmotic stress by suppression subtractive hybridization. Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).