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Efficient plant regeneration from cotyledon-derived protoplasts of cytoplasmic male-sterile tuber mustard (Brassica juncea Coss. var. tumida Tsen et Lee)

Chen Liping, Zhang Mingfang, Hirata Yutaka, Cao Jiashu, Chen Zhujun

Department of Horticulture,College of Agriculture and Biotechnology,Zhejiang University.Hangzhou 310029,Zhejiang；Japan

Abstract

The NAA or 2, 4-D was necessary for callus proliferation. The absence of NAA and 2, 4-D resulted in production of the callus with low regenerative potential, and in the presence of NAA (0.25 mg/L) and 2, 4-D (0.25 mg/L), compact and bright calli were produced with high reproductive potential. Regenerated shoots were obtained at a frequency of nearly 29% when the compact and bright calli were selected and transferred at an early stage to the medium containing BA (1 mg/L) and NAA (0.2 mg/L). All the regenerated shoots were rooted on 1/2 MS medium with NAA 0.1 mg/L added.

Key words: Tuber mustard;Cytoplasmic male-sterile line;Protoplast culture;Regeneration

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Citing This Article：

Chen Liping, Zhang Mingfang, Hirata Yutaka, Cao Jiashu, Chen Zhujun. Efficient plant regeneration from cotyledon-derived protoplasts of cytoplasmic male-sterile tuber mustard (Brassica juncea Coss. var. tumida Tsen et Lee). Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).