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Analysis of influence of Wx intron 1 on gene expression in transgenic rice plant

Cheng Shijun, Ge Hongfei, Wang Zongyang, Hong Mengmin

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Results show that either the deletion of intron 1 or a single base mutation (G→T) at the 5^ splice site of Wx intron 1 decreased the expression of GUS gene. These results indicate that in vivo the intron 1 indeed enhances the gene expression, and the G→T mutation in Wx intron 1 of rice cultivars with intermediate/low amylose content is, probably the key factor causing abnormal splicing of this intron in these cultivars.

Key words: Wx gene;Intron 1;Gene expression

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Citing This Article：

Cheng Shijun, Ge Hongfei, Wang Zongyang, Hong Mengmin. Analysis of influence of Wx intron 1 on gene expression in transgenic rice plant. Acta Physiol Sin 2001; 53 (5): (in Chinese with English abstract).