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Effects of murine bone marrow endothelial cell conditioned medium in combination with FL and TPO on the growth of HPP-CFC and CFU-GM

Na Xiaodong, Wang Qiru

Research Laboratory of Blood Physiology,Hunan Medical University.Changsha 410078,Hunan

Abstract

In the present study, the effects of murine bone marrow endothelial cell conditioned medium (ECM) combined with flt3 ligand (FL) or/and thrombopoietin (TPO) on the proliferation of HPP-CFC and CFU-GM were investigated. Both ECM and the concentrated retentate of ECM (MW>10 kD) promoted the growth of CFU-GM and HPP-CFC, and this promoting effect was further enhanced by addition of FL or TPO. Using reverse transcriptase-polymerase chain reaction (RT-PCR) tchnique, the expression of FL and TPO mRNA was not found in murine bone marrow endothelial cells.

Key words: Bone marrow endothelial cells;Serum-free conditioned medium;Regulation of hematopoiesis;Mice

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Citing This Article：

Na Xiaodong, Wang Qiru. Effects of murine bone marrow endothelial cell conditioned medium in combination with FL and TPO on the growth of HPP-CFC and CFU-GM. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).