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Production of rice plants containing multiple transgenes

Feng Daorong, Xu Xinping, Li Baojian

Biotechnology Research Center,Zhongshan University.Guangzhou 510275,Guangdong

Abstract

We constructed two or three target genes and the marker gene hpt into one vector, and transformed them into one indica rice variety and four japonica rice varieties. Among them, the Zhonghua No.8 had the highest transformation rate 56.4%, and the transformation rate of Qisiruanzhan, Teqing, Qimiaoxiang and Jiuliuling is 6.4%, 2.5%, 2.8% and 1.7%. Molecular analysis proved that 100% of the regenerated plants contained hpt transgenes after hygromycin selection for two times, and more than 70% of them contained intact expression construct of foreign target gene. Genetic analysis showed that the hyg~(r)/hyg~(s) ratio in most of T_(1) plants is near 3 to 1, corresponding to Mendelian ratio.

Key words: Rice;Biolistic;Transgenic rice plants;Molecular analysis

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Citing This Article：

Feng Daorong, Xu Xinping, Li Baojian. Production of rice plants containing multiple transgenes. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).