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Expression of GUS gene in transgenic tobacco regulated by BcNA1 promoter from Brassica napus

Shi Dongqiao, Zhou Yihua, Wan Lihong, Liu Guizhen, Hu Zanmin, Chen Zhenghua

Institute of Genetics,Chinese Academy of Sciences.Beijing 100101

Abstract

The upstream regulatory region of a seed-specific gene was separated from Brassica napus cv. H15 genomic DNA by polymerase chain reaction. Plant expression vector was constructed by ligating this promoter with GUS gene and transferred into tobacco. GUS analysis made on the transgenic plants indicated that the promoter could drive the expression of GUS gene seed-specifically and the activity of GUS changed with the development of seeds. The results of GUS assay also suggested that the genetic transmission of the BcNA1 promoter conforms to Mendelian laws.

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Citing This Article：

Shi Dongqiao, Zhou Yihua, Wan Lihong, Liu Guizhen, Hu Zanmin, Chen Zhenghua. Expression of GUS gene in transgenic tobacco regulated by BcNA1 promoter from Brassica napus. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).