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Preliminary separation and identification of the neurotrophic substances from dorsal spinal cord of chicken embryos

Liu Yaobo, Xue Qingshan, Xiao Yuping, Wang Xianrong

The Institute of Basic Medical Sciences,Academy of Military Medicine.Beijing 100850；China；Department of Biology,Sun Yat-Sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

Extracts of spinal dorsal horn and ventral horn of chicken embryos hatched for 9 days were separated by Sephadex G-75 gel filtration, and different chromatographic fractions of D I and D II, V I and V II were obtained. The neurite-outgrowth promoting effects of the various fractions were assayed by the dorsal root ganglion (DRG) culture in vitro. It was shown that D II, but not D I, fraction of the dorsal horn extract exhibited a significant neurite-outgrowth promoting effect on DRG. By means of SDS-PAGE, the molecular weight of D II fraction ranged from 61 to 15 kD. V I and V II fraction of ventrla horn showed no obvious effect on neurit-outgrowth of DRG in vitro.

Key words: Spinal dorsal horn;Neurotrophic activity;Dorsal root ganglion

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Citing This Article：

Liu Yaobo, Xue Qingshan, Xiao Yuping, Wang Xianrong. Preliminary separation and identification of the neurotrophic substances from dorsal spinal cord of chicken embryos. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).