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An exploration of animal behavior screen platform for novel gene function in central nervous system

Jin Meilei, Mo Yun, Liu Liu, Guo Ning, Xie Qinglian, Lin Zhen, Wang Xing, Li Baoming, Zhao Guoping, Jing Naihe, Yu Lei

Research Center of Biotechnology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences.Shanghai 200233；China；Institute of Biochemistry ＆ Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences.Shanghai 200031

Abstract

For the purpose of large-scale screening of novel gene functions in mammalian nervous system, we have developed an animal behavior-monitoring platform employing antisense-oligo technology. Twenty genes of different categories were chosen from a low abundant gene cDNA sub-library of rat brain. Antisense oligo-nucleotides of these genes were designed and synthesized according to the homologues of the genes in mouse for mouse behavior tests. These antisense oligos were injected into the lateral ventricles of mouse brain using a Hamilton micro-syringe, with saline and oligos of scramble sequences as controls.

Key words: Gene function;Animal behavior;Antisense-oligo;cDNA sub-library;Nervous system

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Citing This Article：

Jin Meilei, Mo Yun, Liu Liu, Guo Ning, Xie Qinglian, Lin Zhen, Wang Xing, Li Baoming, Zhao Guoping, Jing Naihe, Yu Lei. An exploration of animal behavior screen platform for novel gene function in central nervous system. Acta Physiol Sin 2001; 53 (4): (in Chinese with English abstract).