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Activity change and distribution of #beta#-cyanoalanine synthase in rice seeds during germination

Liang Wusheng, Mao Bizeng, Li Shuai, Zhuang Fanglei, Li Debao

Institute of Biotechnology,Zhejiang University.Hangzhou 310029,Zhejiang；China

Abstract

Non-denaturing polyacrylamide gel electrophoresis indicated that two CAS isozymes, named CAS_(1) (with a lower migration rate) and CAS_(2) (with a higher migration rate), occurred in the roots, leaves and endosperm of the rice seedlings. The activities of CAS_(1) were obviously higher than those of CAS_(2) in both roots and endosperm. But the two CAS isozymes occurred with nearly equal activities in leaves. Further studies showed that the two CAS isozymes were both present in leaf cytoplasm, with nearly equal activities. But only CAS_(1) existed in mitochondria and no any CAS existed in chloroplast.

Key words: Rice seed;Germination;#beta#-cyanoalanine synthase;Activity;Distribution

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Citing This Article：

Liang Wusheng, Mao Bizeng, Li Shuai, Zhuang Fanglei, Li Debao. Activity change and distribution of #beta#-cyanoalanine synthase in rice seeds during germination. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).