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A novel method for investigation and differentiation between apoptosis and necrosis of plant cells in suspension culture

Ge Zhiqiang, Yuan Yingjin, Li Jingchuan, Hu Zongding

Department of Biochemical Engineering,Tianjin University.Tianjin 300072

Abstract

The results demonstrate that this method is simpler, quicker and more accurate; moreover, the method overcomes the difficulty of discriminating the cells undergoing apoptosis from the cells undergoing necrosis by the alteration of the cell membrane permeability. Thus, this method is useful in studying on apoptosis of the plant cells.

Key words: Plant cells;Apoptosis;Necrosis;Suspension culture;Fluoresence staining;Enzymic staining

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Citing This Article：

Ge Zhiqiang, Yuan Yingjin, Li Jingchuan, Hu Zongding. A novel method for investigation and differentiation between apoptosis and necrosis of plant cells in suspension culture. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).