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Cloning of homologous targeting sequences containing chloroplast genes, rbcL and atpB, of Brassica napus and the construction of plastid transformation vectors harboring key enzyme genes responsible for PHB biosynthesis

Zhang Zhonglin, Zhang Jingyu, Li Zhinu, Su Ning, Song Yanru, Shen Guifang

Biotechnology Research Institute,Chinese Academy of Agricultural Sciences.Beijing 100081；China

Abstract

The results of restriction analysis and Southern blot hybridization were in accord with the desired. Transformation and consquent works are in progress.

Key words: Phb genes;Chloroplast transformation;Homologous targeting sequence;Site-specific integration;Brassica napus

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Citing This Article：

Zhang Zhonglin, Zhang Jingyu, Li Zhinu, Su Ning, Song Yanru, Shen Guifang. Cloning of homologous targeting sequences containing chloroplast genes, rbcL and atpB, of Brassica napus and the construction of plastid transformation vectors harboring key enzyme genes responsible for PHB biosynthesis. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).