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Effects of different promoters controlling expression of Ac transposase gene on excision frequency of maize Ds element in rice

Chen You, Zhang Jingliu

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Three chimeric genes constructed by fusion of three different promoters, Ac promoter, CaMV 35S promoter and Ubi promoter, to Ac transposase coding region, were each induced into rice calli generated from immature embryo containing Ds element mediated by Agrobacterium. The effects of Ac transposase (Ts) gene expression directed by these three different promoters on the excision frequency of Ds element were compared in rice calli carrying both Ts gene and Ds element. The excision frequency of Ds element trans-activated by Ac transposase driven by Ubi promoter was found to be highest. Nine F_(1) offsprings which had both the Ubipro-Ts gene and the Ds element were obtained by crossing transgenic plants containing Ubipro-Ts gene and rice plant strain 87-1 harboring the Ds element. PCR analysis showed that Ds elements in 8 of these F_(1) plants were excised from their original insertion sites. A DNA fragment flanking the Ds element from a F_(1) plant was cloned using the Inverse-PCR method. Comparison of the nucleotide sequence of this DNA fragment with that at the original site of Ds element in the parent plant strain 87-1 revealed that the Ds element had been excised from the original site an re-integrated into a new genomic site.

Key words: Rice;Promoter;Ac transposase gene;Ds element;Transposition

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Citing This Article：

Chen You, Zhang Jingliu. Effects of different promoters controlling expression of Ac transposase gene on excision frequency of maize Ds element in rice. Acta Physiol Sin 2001; 53 (3): (in Chinese with English abstract).