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Acetylcholine and A23187 stimulate epinephrine secretion of isolated rat adrenal medulla cells in vitro

Liu Yi, Ling Yiping, Yu Zhang, Wu Zhengquan, Zhong Cisheng

Department of Biophysics,School of Basic Medical Sciences,Fudan University.Shanghai 200032

Abstract

Acetylcholine and A23187, two acetylcholinergic agonists, were used to induce the stimulus-secretion coupling of the isolated rat adrenal medulla cells. Morphometry of numerical density and electron probe X-ray quantitative microanalysis were adopted to measure are alterations in the number and calcium content of chromaffin granules in adrenal medulla cells during the agonist treatment. Secretion of epinephrine was detected by the high performance liquid chromatography (HPLC).

Key words: Isolated adrenal medulla cell;Chromaffin granule;Numerical density;Calcium content;Epinephrine

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Citing This Article：

Liu Yi, Ling Yiping, Yu Zhang, Wu Zhengquan, Zhong Cisheng. Acetylcholine and A23187 stimulate epinephrine secretion of isolated rat adrenal medulla cells in vitro. Acta Physiol Sin 2001; 53 (2): (in Chinese with English abstract).