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Differential expression of genes in the pigeon forebrain after removal of the left retina

Xu Lei, Li Xueli, Wang Yao

Department of Biochemistry,School of Medicine Tongji University.Shanghai 200070

Abstract

Suppression subtractive hybridization (SSH) was used to analyze differential expression of genes in the pigeon forebrain after the left retina of the pigeon was removed at hatching. Four genes out of the 14 identified clones produced positive hybridization signals as shown by reverse Northern blot. Sequencing results showed that the length of PFB/SSH-8 is 226 bp, of which 138 bp produced a homology of 85% with human CaMI; the length of PFB/SSH-15 is 252 bp, showing low homology with nexin I alpha; no matched homological information was found in the remaining 2 clones.

Key words: Pigeon;Forebrain;Suppression subtractive hybridization(SSH);Gene expression;Reverse northern blof

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Citing This Article：

Xu Lei, Li Xueli, Wang Yao. Differential expression of genes in the pigeon forebrain after removal of the left retina. Acta Physiol Sin 2001; 53 (2): (in Chinese with English abstract).