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Effects of exogenous ~(1)O_(2) and ·OH and their scavengers on the capacity and operation of the alternative respiratory pathway in tobacco callus

Zhou Gongke, Kong Yingzhen, Li Hongyu, Liang Houguo

The State Key Laboratory of Arid Agroecology,Lanzhou University.Lanzhou 730000,Gansu；China

Abstract

In tobacco (Nicotiana rustica L. Gansu Yellow Flower) callus, addition of exogenous ~(1)O_(2) induced a pronounced increase in the operation activity of alternative respiratory pathway without affecting its capacity. The operation activity of alternative pathway activated by ~(1)O_(2) can be abolished by its scavenger (His). Exogenous ·OH diminished the operation activity and capacity of alternative pathway, which could be recovered by the addition of ·OH scavengers, such as DMSO or MAN. These observations suggest that ~(1)O_(2) is involved in some way in the activating the alternative pathway, but not in the induction of the capacity of alternative pathway; but ·OH can suppress both the actual operation activity and capacity of alternative pathway remarkably. We deduced that ~(1)O_(2) and ·OH might affect the alternative pathway by regulating the activity of the alternative oxidase.

Key words: Tobacco callus;.OH，O_(2);Alternative respiratory path way

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Citing This Article：

Zhou Gongke, Kong Yingzhen, Li Hongyu, Liang Houguo. Effects of exogenous ~(1)O_(2) and ·OH and their scavengers on the capacity and operation of the alternative respiratory pathway in tobacco callus. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).