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Construction of a plant binary expression vector containing intron-kanamycin gene and transformation in Nicotiana tabacum

Peng Rihe, Huang Xiaomin, Li Xian, Sun Aijun, Yao Quanhong, Peng Youliang

Shanghai Key Laboratory of Agricultural Genetic and Breeding,Agro-Biotechnology Research Center,Shanghai Academy of Agricultural Sciences.Shanghai 201106

Abstract

From these results, it can be concluded that the intron-kanamycin gene can be used as an appropriated gene in Agrobacterium-mediated plant transformation.

Key words: Intron-kanamycin gene;GUS activity;Transformation in tobacco

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Citing This Article：

Peng Rihe, Huang Xiaomin, Li Xian, Sun Aijun, Yao Quanhong, Peng Youliang. Construction of a plant binary expression vector containing intron-kanamycin gene and transformation in Nicotiana tabacum. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).