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Male sterile transgenic cabbage plants with TA29-barnase gene

Shen Gezhi, Wang Xinqi, Zhu Yuying, Yang Hongjuan, Lu Guihua, Wang Jiang, Wan Xinshan, Zhang Jingliu

Crop Breeding Institute,Shanghai Academy of Agricultural Sciences.Shanghai 201106；China

Abstract

RNase gene (barnase) was cloned from Bacillus amyloliquefaciens genomic DNA, and the upstream regulatory region (-1 300-+51) of a tapetum-specific gene TA29 was cloned from tobacco (Nicotiana tabacum L.) total DNA by PCR and sequenced. Then a chimeric gene containing the regulatory region of TA29 gene and coding region of barnase gene was constructed and introduced into cabbage with Agrobacterium tumefaciens. Completely sterile, and half-sterile were observed among some transformed cabbages carring TA29-barnase chimeric gene, and other traits were basically identical to unstransformed control plants, but filaments of stamens of transformed plant were shorter than normal ones, anthers were shriveled and without pollen. Self pollination of transformed plants failed to produce fruit capsules and seeds, but hybrid seeds were obtained whtn these plants were cross-pollinated artificially with normal pollen from untransformed cabbage plants. The results showed that these transformed plants were male sterile.

Key words: Cabbage;;;

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Citing This Article：

Shen Gezhi, Wang Xinqi, Zhu Yuying, Yang Hongjuan, Lu Guihua, Wang Jiang, Wan Xinshan, Zhang Jingliu. Male sterile transgenic cabbage plants with TA29-barnase gene. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).