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Cloning of cDNAs encoding calmodulin-binding protein from Arabidopsis cDNA expression library

Li Guoliang, Zhan Hongli, Li Cuifeng, Liu Hua, Ling Qilang, Zhang R

Department of Biochemistry and Molecular Biology,Nankai University.Tianjin 300071

Abstract

Using protein-protein interaction, an Arabidopsis #lambda#ZAP II cDNA expression library was screened with biotinylated calmodulin as a probe. Nine positive clones were isolated. The results of Western blotting of fusion proteins indicated that all these positive clones could synthesize peptides which could bind calmodulin. Eight of them bound calmodulin depending on the presence of Ca~(2+), but only one fusion protein encoded by Y7 cDNA clone, which was the same as CaM BP-10, could bind CaM in a way independent of Ca~(2+).

Key words: Biotinylated;CaM calmodulin binding;Protein expression library screening

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Citing This Article：

Li Guoliang, Zhan Hongli, Li Cuifeng, Liu Hua, Ling Qilang, Zhang R. Cloning of cDNAs encoding calmodulin-binding protein from Arabidopsis cDNA expression library. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).