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Gold particle amount per bombardment of wheat transformation by biolistic method and phenotypic character analysis of transgenic plants and their progenes

An Hailong, Wei Zhiming, Huang Jianqiu

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Effects of gold particle amount pe bombardment (30, 100, 250, 500 #mu#g) on transient and stable transformation of immature wheat embryos, were studied. And 500 #mu#g gold particle per bombardment is superior to others both in GUS transient expression and transgenic plant production. PCR and Southern analyses of T_(0) and T_(1) plants confirmed the integration of foreign bar gene fragments into wheat genomes. T_(1) plants smeared with L-PPT 200 mg/L showed a low level of resistance to this herbicide. The comparison of phenotypic character of T_(0), T_(1), T_(2) and their control plants showed that the phenotype abnormality of T_(0) plants is only a temporary phenomenon due to physiological and cultivated conditions, and the plants will recover from this abnormality partially or fully in the following progenes.

Key words: Particle bombardment;Triticum aestivum L.;Genetic transformation;Phenotypic character

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Citing This Article：

An Hailong, Wei Zhiming, Huang Jianqiu. Gold particle amount per bombardment of wheat transformation by biolistic method and phenotypic character analysis of transgenic plants and their progenes. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).