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The stabilization of plasmid carrying hup genes and the expression of H_(2)-uptake hydrogenase in Klebsiella oxytoca

Huang Li, Liang Jianguang, Wu Yongqiang

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

The resulting transconjugants were analyzed for plasmid stability. Plasmid pKH1 was found to have high stability in its host K. oxytoca. High levels of H_(2)-uptake hydrogenase activity and nitrogen-fixing efficiency relative to the wild type were observed in the two transconjugants NGH999 and NGH982. The increase was more remarkable in a medium with low sucrose content. Transconjugant NGH982 showed partially derepressional expression of nitrogenase in the presence of ammonium.

Key words: Klebsiella oxytoca;Stabilization of Alasmid;H_(2)-uptake hydrogenase activity;Hup gene

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Citing This Article：

Huang Li, Liang Jianguang, Wu Yongqiang. The stabilization of plasmid carrying hup genes and the expression of H_(2)-uptake hydrogenase in Klebsiella oxytoca. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).