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Role of conditioned growth medium for ventricular fibroblasts in promoting fibroblast collagen synthesis and proliferation

Gong Suzhen, Liu Peiqing, Lu Wei, Wang Tinghuai, Fu Shigan, Pan Jingyun

Department of Physiology,Sun Yat-Sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

Ventricular fibroblasts were cultured using conditioned growth medium for ventricular fibroblasts (FCGM). The rate of the total collagen synthesis of ventricular fibroblasts was measured by assaying the incorporation rate of [~(3)H]-proline, whereas the proliferation of ventricular fibroblasts was assessed by determining the incorporation rate of [~(3)H]-TdR and the expression of c-fos genes.

Key words: ventricular fibroblast;［3H］-proline;［3H］-TdR;c-fos mRNA;Heart

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Citing This Article：

Gong Suzhen, Liu Peiqing, Lu Wei, Wang Tinghuai, Fu Shigan, Pan Jingyun. Role of conditioned growth medium for ventricular fibroblasts in promoting fibroblast collagen synthesis and proliferation. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).