Intrathecal injection of NOS antisense oligonucleotides inhibits the increase of NMDA_(1A)R mRNA expression in the spinal cord and brainstem of morphine-withdrawal rats
Cao Junli, Liu Huifen, Zhou Wenhua, Yang Guodong, Zhang Licai, Zeng Yinming
Department of Anesthesiology,Affiliated Hospital of Xuzhou Medical College.Xuzhou 221002,Jiangsu;China;Jiangsu Province Key Laboratory of Anesthesiology.Xuzhou 221002,Jiangsu
Abstract
Methods of reverse transcription polymerase chain reaction (RT-PCR), intrathecal injection and antisense drugs were used to study the effects of nitric oxide (NO) on the scores of morphine-withdrawal syndrome and the expression of NMDA_(1A)R mRNA in rat spinal cord and brainstem. It is suggested that NO mediates morphine-withdrawal reaction and participates in modulating the expression of NMDA_(1A)R mRNA in morphine-withdrawal rats.
Key words: Morphine;withdrawal;NO;NMDA
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Cao Junli, Liu Huifen, Zhou Wenhua, Yang Guodong, Zhang Licai, Zeng Yinming. Intrathecal injection of NOS antisense oligonucleotides inhibits the increase of NMDA_(1A)R mRNA expression in the spinal cord and brainstem of morphine-withdrawal rats. Acta Physiol Sin 2001; 53 (1): (in Chinese with English abstract).