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Role of pp60c-src in mitogen-activated protein kinase activation of vascular smooth muscle cells

Liu Dong, Lu Junsheng, Yin Xiaolong

Department of Cardiology,The Second Affiliated Hospital to Zhejiang University Medical School.Hangzhou 310006,Zhejiang

Abstract

The effect of pp60c-src on mitogen-activated protein kinase (MAPK) activation induced by angiotensin II (Ang II) in vascular smooth muscle cells (VSMCs) was observed to explore the signal transduction process of VSMCs proliferation mediated by Ang II. Following transfection of the synthetic antisense c-src oligodeoxynucleotides (ODNs) wrapped in lipofectin into cultured rat VSMCs, pp60c-src content markedly decreased, pp60c-src kinase activity was only 14.4% of control, while MAPK activity was not significantly changed (P>0.05). When these transfected VSMCs were further stimulated by Ang II, the increase in MAPK activity was only 1.6% of control. These data suggest that pp60c-src is needed for MAPK activation in VSMCs stimulated by Ang II, and pp60c-src may be an important signaling molecule in abnormal proliferation of VSMCs.

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Citing This Article：

Liu Dong, Lu Junsheng, Yin Xiaolong. Role of pp60c-src in mitogen-activated protein kinase activation of vascular smooth muscle cells. Acta Physiol Sin 2000; 52 (6): (in Chinese with English abstract).