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17#beta#-estradiol induced nitric oxide release in vascular endothelial cells

Wang Tinghuai, Yang Dan, Liu Peiqing, Gong Suzhen, Lu Wei, Pan Jingyun

Department of Physiology,Sun Yat-sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

Bovine aortic endothelial cells (BAECs) were used to study the effect of 17#beta#-estradiol (E_(2)) on nitric oxide (NO) release, nitric oxide synthase (eNOS) mRNA expression and intracellular free calcium concentration ([Ca~(2+)]_(i)) and modulation of the effect of E_(2) by estrogen receptor (ER) antagonist tamoxifen and NOS inhibitor L-NAME. E_(2) (10~(-12)-10~(-8) mol/L) induced NO release of BAECs in a concentration-dependent manner and the abundant expression of eNOS mRNA in BAECs increased obviously after treatment with E_(2) (10~(-8) mol/L) for 48 h.

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Citing This Article：

Wang Tinghuai, Yang Dan, Liu Peiqing, Gong Suzhen, Lu Wei, Pan Jingyun. 17#beta#-estradiol induced nitric oxide release in vascular endothelial cells. Acta Physiol Sin 2000; 52 (6): (in Chinese with English abstract).