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mRNA and protein expression of skeletal DHPR_(#alpha#1) and RyRs in diaphragm muscle of rabbits

Zhang Suiyang, Liu Gang, Wang Donglin, Guo Xianjian, Qian Guisheng

Institute of Respiratory Diseases of the New Bridge Hospital,The Third Military Medical University.Chongqing 400037

Abstract

To detect mRNA and protein expression of skeletal dihydropridine receptor isoform alpha_(1) subunit and ryanodine receptor 1 and 3 in diaphragm muscle of rabbits, the coupling mode and characteristics of Ca~(2+) release were explored. Reverse transcription PCR, in situ hybridization and immunohistochemical methods were employed. A higher level of mRNA and protein expression of DHPR_(#alpha#1) and RyR_(1), and a lower level of mRNA expression of RyR_(3) were found. It is suggested that the calcium release unit may consist of skeletal DHPR isoform, RyR_(1) and RyR_(3), and there may be two kinds of Ca~(2+) release mode via conformational changes in linked proteins and Ca~(2+)-induced Ca~(2+) release (CICR) in diaphragm muscle of rabbits.

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Citing This Article：

Zhang Suiyang, Liu Gang, Wang Donglin, Guo Xianjian, Qian Guisheng. mRNA and protein expression of skeletal DHPR_(#alpha#1) and RyRs in diaphragm muscle of rabbits. Acta Physiol Sin 2000; 52 (6): (in Chinese with English abstract).