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Analysis of P/N leak subtraction of pClamp acquisition software

LIU Zhen-Wei, LI Li-Jun, LIU Chuan-Gui

Institute of Pharmacology and Toxicology , Academy of Military Medical Sciences , Beijing 100850, China

Abstract

Using blind whole cell recording technique on rat hippocampal slices, the function and mechanisms of P/N leak subtraction of Clampex program in pClamp acquisition software were studied. The ways of picking up sub-pulse current and properly selecting parameters, including time course, polarization, sub-holding potential, numbers and location of P/N sub-pulse were analyzed in detail. Our results indicate that voltage-gated ion channel currents recorded by Clampex will be more accurate and reproducible by properly using P/N leak subtraction.

Key words: leak subtraction;Patch clamp;hippocampal slice

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Citing This Article：

LIU Zhen-Wei, LI Li-Jun, LIU Chuan-Gui. Analysis of P/N leak subtraction of pClamp acquisition software. Acta Physiol Sin 2000; 52 (5): 440-443 (in Chinese with English abstract).