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Pituitary adenylate cyclase activating polypeptide ameliorates the damage and inhibits the increase of intracellular calcium concentration in cultured hippocampal neurons induced by glutamate

Dong Yan, Tang Tieshan, Lu Changlin, He Cheng, Dong Jianbo, Huang Xiuying, Sun Fangzhen, Bao Xuan

Department of Neurobiology,Second Military Medical University.Shanghai 200433；China；Shanghai Institute of Brain Research,Chinese Academy of Sciences.Shanghai 200031

Abstract

Hippocampal neurons cultured from 7 to 9 d in vitro were used to observe the effect of glutamate. Treatment of glutamate for 24 h greatly decreased neuronal survival and pretreatment with pituitary adenylate cyclase activating polypeptide (PACAP) significantly atenuated hippocampal neuron death induced by glutamate. Moreover, glutamate dose-dependently increased the intracellular calcium concentration in cultured hippocampal neurons, while PACAP inhibited the increase of intracellular calcium concentration induced by glutamate. PACAP 6-38, a specific PACAP type I receptor antagonist, completely inhibited the amelioration of glutamate induced death and the decrease of intracellular calcium concentration induced by PACAP in cultured hippocampal neurons.

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Dong Yan, Tang Tieshan, Lu Changlin, He Cheng, Dong Jianbo, Huang Xiuying, Sun Fangzhen, Bao Xuan. Pituitary adenylate cyclase activating polypeptide ameliorates the damage and inhibits the increase of intracellular calcium concentration in cultured hippocampal neurons induced by glutamate. Acta Physiol Sin 2000; 52 (5): 402-406 (in Chinese with English abstract).