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Isolation of regulation genes related with liver regeneration by representational difference analysis

Xu Wangxiang, Wang Siying, Wei Handong, Yang Xiaoming

Institute of Radiation Medicine,Academy of Military Medical Sciences.Beijing 100850

Abstract

mRNA isolated from 2/3 partially hepatectomied rats was used as a "tester" for representational difference analysis (RDA). The subtracted tester cDNA was cloned into a T-vector and a rat regeneration liver-specific EST pool was constructed, which contained about 3×10~(4) independent clones. A sequence analysis of 52 clones randomly picked up from this pool indicated that the liver regeneration specific sequences were enriched, and the results of RNA blots revealed some novel genes in association with liver regeneration.

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Citing This Article：

Xu Wangxiang, Wang Siying, Wei Handong, Yang Xiaoming. Isolation of regulation genes related with liver regeneration by representational difference analysis. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).