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Signal transduction pathway for antral smooth muscle cell contraction induced by motilin and gastrin in rats

Wang Ling, Zhou Lu

Department of Physiology,Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College.Beijing 100005

Abstract

Signal transduction pathway that mediates motilin- and gastrin-induced contraction of antral smooth muscle cells was investigated in rats. The results are as follows. (1) Motilin and gastrin induced contraction of isolated gastric smooth muscle cells in a dose-dependent manner. (2) Motilin- and gastrin-induced antral smooth muscle cell contraction was inhibited by antibodies against G_(ai-3). Motilin and gastrin caused an increase of Guanosine-5^-(3-[~(35)S]thio) triphosphate ([~(35)S]GTP#gamma#S) binding of G_(ai-3). (3) Contraction of antral muscle cells induced by motilin and gastrin was inhibited by the phospholipase C (PLC) inhibitor U-73122 and the IP_(3) receptor antagonist heparin.

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Citing This Article：

Wang Ling, Zhou Lu. Signal transduction pathway for antral smooth muscle cell contraction induced by motilin and gastrin in rats. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).