Issue Archive

Responses induced by dopamine in Xenopus laevis oocytes injected with mRNA from heat injured rat striatum

Zhao Yongqi, Zhang Binglie, Wang Luming, Xing Cheng, Li Min, Fan Ming

Institute of Basic Medical Sciences,Academy of Military Medical Sciences.Beijing 100850；China

Abstract

Poly(A)~(+) mRNA isolated from the striatum of normal rats and heat injured rats by RNAgents Total RNA Isolation System and PolyTract mRNA RNAgents Isolation Systems was microinjected into Xenopus laevis ocytes to express functional neurotransmitter receptors. Membrane currents were recorded by the conventional patch clamp technique for measuring the relative amounts of mRNA encoding dopamine receptor in order to study the change in dopamine receptors in heat injured rat striatum. There was no change in resting membrane potentials of the oocytes before and after mRNA injection. Dopamine induced membrane currents were shown to be mediated through Cl~(-). The oocytes injected with mRNA from heat injured rat striatum were less sensitive to dopamine than those injected with mRNA from normal rats (P<0.05).

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Zhao Yongqi, Zhang Binglie, Wang Luming, Xing Cheng, Li Min, Fan Ming. Responses induced by dopamine in Xenopus laevis oocytes injected with mRNA from heat injured rat striatum. Acta Physiol Sin 2000; 52 (4): (in Chinese with English abstract).