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[Effect of Kv1.3 and KCa3.1 potassium ion channels on the proliferation and migration of monocytes/macrophages.] [Article in Chinese]

ZHANG Shuang-Xia, WANG Xian-Pei, GAO Chuan-Yu, JU Chen-Hui, ZHU Li-Jie, DU Yi-Mei

Department of Cardiology, Zhengzhou University People’s Hospital, Zhengzhou 450003, China； Research Center of Ion Channelopathy, Institute of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430000, China

Abstract

This study was aimed to investigate the effects of blockade of Ca2+ activated channel KCa3.1 and voltage-gated potassium channel Kv1.3 of the mononuclear/macrophages on inflammatory monocyte chemotaxis. Chemotaxis assay was used to test the inflammatory Ly-6Chi monocyte chemotaxis caused by the mononuclear/macrophages. The proliferation of mononuclear macrophages was detected by cell counting kit-8 (CCK8). Enzyme-linked immunosorbent assay (ELISA) was applied to detect the C-C motif ligand 7 (CCL7) in cultured media. The results showed that the proliferation of mononuclear/macrophages and the recruitment of Ly-6Chi monocyte induced by mononuclear/macrophages were suppressed by the potent Kv1.3 blocker Stichodactyla helianthustoxin (ShK) and the specific KCa3.1 inhibitor TRAM-34. Meanwhile the secretion of CCL7 from mononuclear/macrophages was decreased. The response of Ly-6Chi monocyte pretreated with ShK or TRAM-34 to CCL2 was declined. These results suggest that KCa3.1 and Kv1.3 may play an important role in mononuclear macrophages’ proliferation and migration.

Key words: mononuclear/macrophages; KCa3.1 potassium ion channel; Kv1.3 potassium ion channel; Ly-6Chi monocytes

Received: 2015-03-14　　Accepted: 2015-05-20

Corresponding author: 高传玉　　E-mail: gaocy6802@163.com

Citing This Article：

ZHANG Shuang-Xia, WANG Xian-Pei, GAO Chuan-Yu, JU Chen-Hui, ZHU Li-Jie, DU Yi-Mei. [Effect of Kv1.3 and KCa3.1 potassium ion channels on the proliferation and migration of monocytes/macrophages.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (5): 505-512 (in Chinese with English abstract).