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[Suppression of E3 ubiquitin ligase Cbl-b in interleukin-1 signaling.] [Article in Chinese]

YU Jiang-Tian, BU Xin, ZHAO Hu, SU Jin^*

Department of Biochemistry and Molecular Biology, The Fourth Military Medical University, Xi’an, 710032

Abstract

The present study aims to investigate the effect of Cbl-b, a member of E3 ubiquitin ligase family, on interleukin-1 (IL-1) pathway in synoviocytes. The protein expression levels of Cbl-b and IL-1-induced matrix metalloproteinase 13 (MMP-13) in synoviocytes were analyzed by Western blot. Collagen substrates were incubated with the conditioned medium collected from synoviocytes cultures and then subjected to SDS-PAGE for analysis of collagen degradation. The results showed that compared with wild-type cells, Cbl-b-deficient cells expressed more MMP-13 protein and had enhanced ability to degrade collagens under IL-1 stimulation. These data suggest that Cbl-b may negatively regulate IL-1-triggered degradation of collagen matrix in synoviocytes.

Key words: Cbl-b; ubiquitin ligase; interleukin-1; matrix metalloproteinase; synoviocytes

Received: 2015-06-15　　Accepted: 2015-07-08

Corresponding author: 苏金　　E-mail: sujin923@fmmu.edu.cn

Citing This Article：

YU Jiang-Tian, BU Xin, ZHAO Hu, SU Jin. [Suppression of E3 ubiquitin ligase Cbl-b in interleukin-1 signaling.] [Article in Chinese]. Acta Physiol Sin 2015; 67 (4): 409-412 (in Chinese with English abstract).