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Influence of hepatic stimulator substance and p21~(ras) expression in human hepatic carcinoma cells BEL-7402

Dai Jie, An Wei, Gao Dingcheng, Chen Li

Department of Cell Biology,Capital University of Medical Sciences.Beijing 100054

Abstract

The hepatic stimulator substance (HSS) was partially purified from schedule-lighted weanling rat livers. The effects of HSS and p21~(ras) expression of human hepatic carcinoma cell BEL-7402 were examined. The results showed: (1) HSS was capable of promoting the proliferation of the cells, and its molecular weight was 14-20 kD; (2) the p21~(ras) expression was increased under HSS effect time-dependently, and the effect of HSS appeared synergistic with EGF; (3) the p21~(ras) expression induced by HSS manifested a dose-dependent manner, and the saturation effect of HSS occurred at the concentration of 100 #mu#g/ml. Considering together with our previous report about HSS regulation on EGF receptor, these results strongly suggest that the proliferatory effect of HSS on hepatic carcinoma cells is presumedly related with eGF receptor-mediated signal transduction.

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Citing This Article：

Dai Jie, An Wei, Gao Dingcheng, Chen Li. Influence of hepatic stimulator substance and p21~(ras) expression in human hepatic carcinoma cells BEL-7402. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).