Issue Archive

Interleukin-2 stimulates the proliferation of cultured RC-4B/C pituitary adenoma cell line

Wang Gaofeng, Zhu Yunlong, Chen Jiankang, Zhang Wanhui, Zhong Yanqing, Hu Yuzhen, Wang Fuzhou

The Fourth Military Medical University.Xi^an 710032,Shaanxi

Abstract

IL-2 (10-1 000 U/ml) significantly stimulated ~(3)H-TdR incorporation into the cell line in a dose-dependent manner. Specific PTK inhibitor tyrphostin (1 #mu#mol/L) suppressed RC-4B/C cell proliferation and blocked the effect of IL-2 on RC-4B/C cells. After the PKA signaling pathway was inhibited by specific PKA inhibitor H-9 (1#mu#mol/L), the proliferation rate of RC-4B/C cells increased significantly. H-9 also enhanced the stimulation of IL-2 on RC-4B/C cell growth. Anti-estrogen tamoxifen (1#mu#mol/L) had no significant effect on the action of IL-2 on the proliferation of RC-4B/C cells.

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Wang Gaofeng, Zhu Yunlong, Chen Jiankang, Zhang Wanhui, Zhong Yanqing, Hu Yuzhen, Wang Fuzhou. Interleukin-2 stimulates the proliferation of cultured RC-4B/C pituitary adenoma cell line. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).