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Plasmid pcDNA3 enhances Ca~(2+) transport of sarcoplasmic reticulum in ischemic skeletal muscle of rats

Jiang Zhisheng, Zhao Wen, Yang Yongzong, Tang Chaoshu, Tang Jian, Jia Hongti, Liu Naikui

Research Institute of Cardiovascular Diseases,Hengyang Medical College.Hengyang 421001；Institute of Cardiovascular Research,Beijing Medical University.Beijing 100083；China

Abstract

The effects of plasmid pcDNA3 on Ca~(2+) transport of sarcoplasmic reticulum (SR) in ischemic skeletal muscle was investigated. The results show that Ca~(2+) transport (including Ca~(2+) uptake and Ca~(2+) release) rate of SR in ischemic skeletal muscle was markedly increased compared with that in non-ischemic muscle (P<0.01 or P<0.05). After plasmid pcDNA3 bound to the DNA binding proteins of SR, Ca~(2+) transport of SR was further increased. The results suggest that the effect of plasmid DNA on the ability of Ca~(2+) transport in SR of ischemic skeletal muscle is the same as is observed in normal skeletal muscle. The pathophysiological significance of the present finding deserves further exploration.

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Citing This Article：

Jiang Zhisheng, Zhao Wen, Yang Yongzong, Tang Chaoshu, Tang Jian, Jia Hongti, Liu Naikui. Plasmid pcDNA3 enhances Ca~(2+) transport of sarcoplasmic reticulum in ischemic skeletal muscle of rats. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).