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The roles of the activities of plasmalemma and tonoplast H~(+)-ATPase on salt-induced proline accumulation of Ficus carica cells

Wang Liangju, Liu Youliang, Ma Kai

Department of Agronomy,Nanjing Agricultural University.Nanjing 210095,Jiangsu

Abstract

Salt stress significantly decreased the pH values of the medium in rotated culture of Ficus carica cells, which could be inhibited by the addition of Na_(3)VO_(4), an inhibitor of plasma membrane H~(+)-ATPase, suggesting that salt-induced pH decrease is through the activation of plasma membrane H~(+)-ATPase. NaCl treatment promoted the plasma membrane H~(+)-ATPase activity of Ficus carica cells, but inhibited the plasma membrane vesicle H~(+)-ATPase activity. Na_(3)VO_(4) completely inhibited salt-induced proline accumulation at a concentration of 50 #mu#mol/L, but to a lesser degree at higher concentrations, implying that there is no simple direct relationship between the accumulation of proline and plasmalemma H~(+)-ATPase activity. Na_(3)VO_(4) at higher concentrations also promoted tonoplast H~(+)-ATPase activity, implying that the loss of plasma membrane H~(+)-ATPase caused by Na_(3)VO_(4) was offset by promotion of the tonoplast H~(+)-ATPase activity, which increased cytosol pH and thereby promoted proline accumulation. High pH of cultural medium, probably favoring the pumping out of H~(+) from cytosol, promoted proline accumulation under salt or no-salt treatment.

Key words: Salt stress;Ficus carica L.;Plasma membrane H~(+)-ATPase;Tonoplast H~(+)-ATPase;Proline accumulation

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Citing This Article：

Wang Liangju, Liu Youliang, Ma Kai. The roles of the activities of plasmalemma and tonoplast H~(+)-ATPase on salt-induced proline accumulation of Ficus carica cells. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).