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Regeneration of transgenic cucumber plants with a WMV-2 CP gene

Wang Huizhong, Zhao Peijie, Zhou Xiaoyun

Department of Biology,Hangzhou Normal College.Hangzhou 310036,Zhejiang；China

Abstract

Cotyledon segments of Cucumis staivus L. were used as explants for transformation mediated by Agrobacterium tumefaciens strain LBA4404 harboring the binary vector pBPMWMV containing an npt-II gene and a WMV-2 CP gene. Transgenic plants were obtained and segregation of WMV-2 CP gene in R1 transgenic plants was analysed. Successful genetic transformation was confirmed by DNA dot hybridization, PCR assay and Southern blot analysis. The incidence of virus disease was low and late on transgenic plants, thus showing that it had a certain resistance to relevant viruses.

Key words: Cucumis sativus L.;Cotyledon;Agrobacterium tumefaciens;CP gene;Transgenic plant;Resistance to virus

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Citing This Article：

Wang Huizhong, Zhao Peijie, Zhou Xiaoyun. Regeneration of transgenic cucumber plants with a WMV-2 CP gene. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).