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Studies on the splicing ability of Wx gene intron 1 in glutinous rice

Ge Hongfei, Wang Zongyang, Hong Mengmin

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Two chimeric plasmids with Wx gene promoter (from O. sativa subsp. indica cv. 232)、 Wx first intron (from O. sativa subsp. indica cv. 232 or subsp. japonica cv. Hanfeng) and coding region of GUS reporter gene were constructed, then they were introduced into glutinous rice O. sativa subsp. japonica cv. Fengnuo 593. These two chimeric genes were also introduced into nonglutinous indica rice Teqing and japonica rice Zhonghua11 to serve as control. The GUS activities in the transformed calli and the immature seeds of transgenic plants were determined.

Key words: Glutinous rice;Wx gene;Intron 1 gene;Expression

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Citing This Article：

Ge Hongfei, Wang Zongyang, Hong Mengmin. Studies on the splicing ability of Wx gene intron 1 in glutinous rice. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).