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Study on mitosis and chromosome stability of callus of Taxus cuspidata

Wang Shuhao, Qi Huaifeng, Yuan Yingjin

Department of Biochemical Engineering,College of Chemical Engineering,Tianjin University.Tianjin 300072

Abstract

Cell division and chromosome number stability of callus of Taxus cuspidata after subculture for 4 years have been studied. Chromosome morphology during mitosis after 3-4 d of culture have been observed. Amitosis did not occur. Chromosome counting showed that the chromosome ploidy of callus cells of T. cuspidata was very stable during subculture for 4 years. The karyotype formula is 2n=24, which agrees with that from root-tip squash of seedlings.

Key words: Taxus cuspidata;Callus;Cell division;Chromosome number;Karyotype

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Citing This Article：

Wang Shuhao, Qi Huaifeng, Yuan Yingjin. Study on mitosis and chromosome stability of callus of Taxus cuspidata. Acta Physiol Sin 2000; 52 (3): (in Chinese with English abstract).