Issue Archive

Isolation of GAE6-3A 5^-upstream fragment from Gossypium arboreum and its expression in tobacco

Yu Xiaohong, Zhu Yongqing, Lin Zhiping, Chen Xiaoya, Xu Zhihong

National Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Digestion with several restrictive enzymes showed that the insert is about 8.0 kb long, containing a 1.5 kb E6 5^-upstream region. Sequence alignment of G. arbireym E6 upstream fragment with upstream fragment 599 bp of an E6 gene (EMBL accession number I34396) revealed a 94.5% identity. The GAE6-3A upstream fragment was subcloned and ligated to the GUS reporter gene, then transferred into tobacco (Nicotiana tabacum) plants by Agrobacterium tumfacien mediated transformation. GUS activity was observed in the epidermis and vascular tissues of leaves, roots and stems.

Key words: GAE6-3A;Upstream fragment;Gossypium arboreum;Cotton fiber

Received: 　　Accepted:

Corresponding author: 　　E-mail:

Citing This Article：

Yu Xiaohong, Zhu Yongqing, Lin Zhiping, Chen Xiaoya, Xu Zhihong. Isolation of GAE6-3A 5^-upstream fragment from Gossypium arboreum and its expression in tobacco. Acta Physiol Sin 2000; 52 (2): (in Chinese with English abstract).