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Role of M-type receptor in internal calcium release and quantal secretion in rat adrenal chromaffin cells

Xu Jianhua, Zeng Xuhui, He Liming, Qu Anlian, Zhou Zhuan

Institute of Biophysical Biochem,Huazhong University of Science and Technology.Wuhan 430074,Hubei

Abstract

In rat single adrenal chromaffin cells, the effects of methacholine (MCh) on [Ca~(2+)]_(i) and catecholamine secretion were studied with fura-2 fluorescence and carbon fiber electrodes. In the presence of 2 mmol/L Ca~(2+) in the bath, locally applying 1 mmol/L MCh, either containing or not containing Ca~(2+), evoked both [Ca~(2+)]_(i) and secretion signals. In the absence of Ca~(2+) in the bath, MCh could still evoke [Ca~(2+)]_(i) and secretion.

Key words: Chromaffin cell;mACh-receptor;Ca store;Secretion;Carbon fiber electrode

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Citing This Article：

Xu Jianhua, Zeng Xuhui, He Liming, Qu Anlian, Zhou Zhuan. Role of M-type receptor in internal calcium release and quantal secretion in rat adrenal chromaffin cells. Acta Physiol Sin 1999; 51 (5): (in Chinese with English abstract).