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Ciliary neurotrophic factor prevents NO mediated cytotoxicity in cultured hippocampal neurons

Chen Xiuqing, Chen Zheyu, Lu Changlin, He Cheng, Wang Chenghai, Bao Xuan

Department of Neurobiology,Institute of Neuroscience Research,Second Military Medical University.Shanghai 200433；China

Abstract

The neurotoxicity was induced by NO donors, such as sodium nitroprusside (SNP), s-nitroso-N-acetylpenicillamine (SNAP), NOS substrate, L-arginine (L-Arg) and ionomycin (a calcium ionophore). Preincubation of the cultures with ciliary neurotrophic factor (CNTF) for 24 h promoted neuronal survival and decreased the LDH efflux and the level of NO induced by ionomycin. The protective effects of CNTF against NO cytotoxicity were concentration-dependent.

Key words: Ciliary neurotrophic factor (CNTF);Hippocampal neurons;Nitric oxide (NO);Cytotoxicity

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Citing This Article：

Chen Xiuqing, Chen Zheyu, Lu Changlin, He Cheng, Wang Chenghai, Bao Xuan. Ciliary neurotrophic factor prevents NO mediated cytotoxicity in cultured hippocampal neurons. Acta Physiol Sin 1999; 51 (5): (in Chinese with English abstract).