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Effects of ascorbic acid on membrane damage and activity of some related enzymes in Astragalus adsurgens protoplasts during their isolation and culture

Luo Jianping, Gu Yuehua, Wang Shengbing, Liu Jin

School of Life Sciences,University of Science and Technology of China.Hefei 230026,Anhui

Abstract

These results suggested that the improvement of A. adsurgens protoplast culture by ASA was due to the enhancement of protoplast tolerance to oxidative stress occurring during isolation and culture of protoplasts and that SOD and APX rather than CAT might be the main enzymes responsible for scavenging the reactive oxygen species produced.

Key words: Astragalus adsuegens Pall.;Protoplast;Ascorbic acid (ASA);Malondialdehyde (MDA);Superoxide dismutase (SOD);Ascorbate peroxidase(APX)

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Citing This Article：

Luo Jianping, Gu Yuehua, Wang Shengbing, Liu Jin. Effects of ascorbic acid on membrane damage and activity of some related enzymes in Astragalus adsurgens protoplasts during their isolation and culture. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).