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Cloning and sequencing of 5^-end cDNA fragment of early response gene (ER1) of rice induced by the blast fungus Magnaporthe grisea

Zhou Jianming, Zhu Qun, Bai Yongyan

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032；USA

Abstract

The 5^-end cDNA fragment (ER1^) of early responsive gene (ER1) of rice (Oryza sativa L. subsp. japonica No. 4) induced by blast fungus M. grisea has been isolated and sequenced (606 bp) by 5^ RACE technique. DNA sequence analysis showed that ER1^ (606 bp) can encode 134 amino acids and there are 309 bp nucleotides located at the 3^-end of the untranslated region of mRNA of ER1 gene. The 89 bp overlapping fragment was found between the 5^-end of ER1 cDNA fragment and the 3^-end of ER1^. The partial indentities of amino acid sequence deduced from ER1^ with an unknown protein (gene accession number AC002409) of Arabidopsis thaliana was 82%.

Key words: Rice;Magnaporthe grisea;ER1 gene;5' end cDNA (ER1');5'RACE

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Citing This Article：

Zhou Jianming, Zhu Qun, Bai Yongyan. Cloning and sequencing of 5^-end cDNA fragment of early response gene (ER1) of rice induced by the blast fungus Magnaporthe grisea. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).