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Expression of a rice cDNA c73 in E. coli cells and purification of its fusion protein

Tang Weihua, Qiu Zilong, Wang Zongyang, Zhang Jingliu, Hong Mengmin

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

A rice cDNA fragment c73, one of the candidates for the sequence encoding a DNA-binding protein, has been isolated from a rice cDNA library, using a 31 bp DNA fragment in the 5^-upstream region of rice waxy gene as a probe. To isolate and purify the gene product, the c73 DNA was cloned into a His_(6)-fusion expression vector pET28-c(+). After being transformed into E. coli BL21(DE3), the expected fusion protein encoded by c73 fragment was expressed by induction by IPTG, and purified using Ni-NTA affinity chromatography.

Key words: Rice;Transcription factor;DNA-binding protein;His_(6) fusion expression

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Citing This Article：

Tang Weihua, Qiu Zilong, Wang Zongyang, Zhang Jingliu, Hong Mengmin. Expression of a rice cDNA c73 in E. coli cells and purification of its fusion protein. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).