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Agrobacterium-mediated transformation of rice and the phenotypic characteristics of the transgenic R_(1) plants

Zhang Bing, Wei Zhiming

Shanghai Institute of Plant Physiology,Chinese Academy of Sciences.Shanghai 200032

Abstract

Genetic transformation mediated at Agrobacterium tumeficiens has been applied to two japonica varieties and four indica varieties of rice (Oryza sativa L.). The frequencies of transgenic calli are high (20.83%-62.32%) for both the japonica and indica varieties tested. The frequency of transgenic plants in japonica variety "Shenxiangjing No. 4" was 17.39%, while that in japonica variety "Qiufeng" was 9.21%. Among the 4 indica varieties, only 1 transgenic plant was obtained in "Chaofengzao No. 1". Integration of foreign genes into the genome of transgenic plants was confirmed by Southern analysis, GUS assay (Plate I-b, e) and boundary sequence analysis of T-DNA in transgenic rice plant.

Key words: Agrobacterium tumeficiens;Oryza sativa L.;Genetic transformation;Phenotypic characteristics

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Citing This Article：

Zhang Bing, Wei Zhiming. Agrobacterium-mediated transformation of rice and the phenotypic characteristics of the transgenic R_(1) plants. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).