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Effect of ET-1 on intracellular free calcium in cultured neonatal myocardial cells

Wang Tinghuai, Wu Bin, Zhu Xiaonan, Pan Jingyuan

Department of Physiology,Sun Yat-sen University of Medical Sciences.Guangzhou 510089,Guangdong

Abstract

In this present study, the effects of ET-1 on intracellular free calcium concentration ([Ca~(2+)]_(i)) and the underlying mechanisms were investigated in cultured neonatal rat myocardail cells loaded with fura-2/AM. The results are as follows. ET-1 induced an increase of [Ca~(2+)]_(i) in a dose-dependent manner, which consisted of transient and sustained phase. BQ123, a selective ET_(A) receptor antagonist, blocked the ET-1 induced [Ca~(2+)]_(i) responses, suggesting that these responses were mediated by ET_(A) receptors.

Key words: Endothelin-1;Intracellular free calcium concentration;Pertussis toxin;Nifedipine;Phorbol ester

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Citing This Article：

Wang Tinghuai, Wu Bin, Zhu Xiaonan, Pan Jingyuan. Effect of ET-1 on intracellular free calcium in cultured neonatal myocardial cells. Acta Physiol Sin 1999; 51 (4): (in Chinese with English abstract).